Contribution information

Title Enzymatic synthesis of Cephalexin in an aqueous two phase system environment
Status Accepted
Final type Poster
Final session Bioengineering and biotechnology
Authors L., Vobecká1, Z., Slouka2, M., Přibyl3
1 University of Chemistry and Technology Prague, Praha 6, Czechia
2 UCT Prague, Prague, Czechia
3 University of Chemistry and Technology, Prague, Praha 6, Czechia
Uploaded abstract link
Brief content Cephalexin is a potent antibiotic produced in an enzymatic reaction catalyzed by penicillin acylase. The reaction is characterized by the appearance of a maximum cephalexin concentration. This is given by the reaction reversibility and the consumption of one of the reactants in a side reaction. To avoid cephalexin loss, we carried out the cephalexin synthesis in a kinetically controlled regime in an aqueous two-phase system (ATPS). The advantage of using an ATPS lies in the continuous product withdrawal and the possibility of valuable enzyme recycling.
ID 454