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Title Optimization of the procedure for determination of lead and cadmium species by HPLC/ICP-MS in herbs of Chinese origin
Status Accepted
Final type Poster
Final session Separation processes
Authors E., Koko1, A., Sajnóg2, D., Barałkiewicz3
1 Adam Mickiewicz University in Poznan, Poznan, Poland
2 Adam Mickiewicz University in Poznan, Poznan, Poland
3 Adam Mickiewicz University in Poznan, Poznan, Poland
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Brief content The aim of optimization of the procedure for simultaneous determination was to select speciation forms of lead: Pb2+, (CH3)3Pb+ , and cadmium Cd2+ in reference material. The optimization of the parameters of the chromatographic apparatus will consist in: (i) selecting an analytical column appropriate for the HPLC method, (ii) preparing a mobile phase consisting of a buffer and an organic modifier with a specific pH, (iii) selecting the flow rate of the mobile phase. Optimizing the mass spectrometer for maximum intensities will consist of: (i) setting the torch and coil positions, (ii) setting the plasma power and sample gas velocity, (iii) the voltage on the ion lens, (iv) setting the reaction gas flow in the DRC chamber and the exclusion parameters RPq. Obtained analytical signals for species separation were symmetrical. The applied mobile phase consisted of 40 mM of EDTA, 20 mM of TMAH, 10 of mM oxalic acid, and 16 mM of ammonia. Full separation species was achieved in 2 minutes with use of an isocratic elution program. The next step in the research will be to apply the designed procedure to the analysis of Chinese herb samples.
ID 289