Contribution information

Title Extraction of phenolic compounds from agro-industrial wastes and evaluation of their antioxidative potential
Status Not decided yet
Type Poster
Session Bioengineering and biotechnology
Authors S., Jakovetić Tanasković1, N., Šekuljica2, J., Jovanović3, I., Gazikalović4, A., Stefanović5, S., Grbavčić6, N., Luković7, Z., Knežević-Jugović8
1 Faculty of Technology and Metallurgy, Karnegijeva 4, Belgrade; Serbia, Belgrade, Serbia
2 Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, Belgrade; Serbia, Belgrade, Serbia
3 Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia
4 Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia
5 Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia
6 Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade; Serbia, Belgrade, Serbia
7 Faculty of Technology and Metallurgy, Karnegijeva 4, Belgrade; Serbia, Belgrade, Serbia
8 Faculty of Technology and Metallurgy, Belgrade, Serbia
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Brief content The constant world population growth imposes the need for the production of more food, and as a consequence, has an increase in the organic-rich wastes. These agro-industrial wastes represent the problem from the environmental viewpoint, and their utilization as the raw materials for the production of value-added products has become one of the main topics of biorefinery. In this paper, we used soybean meal, co-product of dietary oil production, as a model waste for optimization of phenolic compounds extraction. Ethanol influenced microwave-assisted extraction is chosen since it is recognized as environmentally friendly and food safe. Optimization of the extraction included finding of optimal liquid/solid ratio, extraction duration, and the microwave power. Under optimized conditions (liquid/solid ratio of 15 ml/g for 70 s at microwave power of 300 W) 3.87 mg GAE/g was extracted from soybean meal, what represented substantial increase compared to 2.46 mg GAE/g obtained before optimization. The same method was further applied for the extraction of phenolics from apple pomace, apple pomace mixed with soybean meal, ultrasound treated soybean meal and, wheat bran. Antioxidant potential of all extracts was evaluated using fixed reaction time DPPH method as well as steady state measurement DPPH method. ABTS and FRAP methods were applied as well. Results showed that extracts had different behavior towards different radicals which was dependent on the type of the phenolics extracted. Apple pomace extract was the most potent towards DPPH radical, while the most effective extract towards ABTS radical was obtained from apple pomace and soybean meal mixture. Soybean meal extract showed the highest reducing power assessed via FRAP method.
ID 270